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Image Search Results
Journal: PLoS ONE
Article Title: Collagenase mRNA Overexpression and Decreased Extracellular Matrix Components Are Early Events in the Pathogenesis of Emphysema
doi: 10.1371/journal.pone.0129590
Figure Lengend Snippet: Antibodies used in immunohistochemistry studies.
Article Snippet: The following primary antibodies were used in this study: a
Techniques: Immunohistochemistry, Plasmid Preparation
Journal: Scientific Reports
Article Title: Effects of induction and inhibition of matrix cross-linking on remodeling of the aqueous outflow resistance by ocular trabecular meshwork cells
doi: 10.1038/srep30505
Figure Lengend Snippet: BAPN (cross-link inhibitor) or genipin (cross-link inducer) were added at time point “0” to ( a ) porcine and ( b ) human anterior segments in perfusion culture. Outflow rates were monitored for a further 69-75 hours. Average outflow facilities at 75 hours after treatment were 0.44 (control), 0.494 (BAPN) and 0.159 (genipin) μl/min/mm Hg for porcine anterior segments and 0.365 (control), 0.489 (BAPN) and 0.321 (genipin) μl/min/mm Hg for human anterior segments. Error bars are the standard error of the mean. *p < 0.05; **p < 0.01. ( c ) Masson’s trichrome histological staining of human TM tissue post-perfusion at 20× and 100×. Immunostaining with fibronectin (red) and fibrillin-1 antibodies (green) are also shown. The cornea is oriented toward the left of each image and the sclera at the right. SC = Schlemm’s canal. Scale bars for immunostained images = 20 μm.
Article Snippet: Membranes were probed with one or more of the following primary antibodies: MMP14 rabbit polyclonal (ab38971; Abcam, Cambridge, MA), a MMP14 mouse monoclonal (IM57; EMD Millipore, Billerica, MA), MMP2 mouse monoclonal (MAB3308; EMD Millipore), TIMP2 rabbit polyclonal (ab2965; EMD Millipore), collagen type I mouse monoclonal (M-38-c; Developmental Studies Hybridoma Bank, Iowa City, IA), CD44 rat monoclonal (60068; StemCell Technologies, Inc., Vancouver, BC), tenascin C rabbit polyclonal (AB19011; EMD Millipore), fibronectin rabbit polyclonal (ab2413; Abcam), elastin mouse monoclonal (ab9519; Abcam),
Techniques: Staining, Immunostaining
Journal: Scientific Reports
Article Title: Effects of induction and inhibition of matrix cross-linking on remodeling of the aqueous outflow resistance by ocular trabecular meshwork cells
doi: 10.1038/srep30505
Figure Lengend Snippet: ( a ) Quantitative RT-PCR of mRNA for various ECM molecules in TM cells treated for 24 hours with BAPN or genipin. Results are shown as a percentage of their respective controls. Data are average ± standard error of the mean. N = 4–6; *p < 0.05. ( b ) Representative Western immunoblots for CD44, fibronectin, fibrillin-1, tenascin C, elastin and versican produced by TM cells treated for 48 hours with BAPN or genipin. Serum-free (SF) control is the vehicle for BAPN and DMSO is the control for genipin. ( c ) Densitometry of each of the bands relative to its control. When multiple bands were present, the strongest band was evaluated. Data are average ± standard error of the mean. N = 3; *p < 0.05.
Article Snippet: Membranes were probed with one or more of the following primary antibodies: MMP14 rabbit polyclonal (ab38971; Abcam, Cambridge, MA), a MMP14 mouse monoclonal (IM57; EMD Millipore, Billerica, MA), MMP2 mouse monoclonal (MAB3308; EMD Millipore), TIMP2 rabbit polyclonal (ab2965; EMD Millipore), collagen type I mouse monoclonal (M-38-c; Developmental Studies Hybridoma Bank, Iowa City, IA), CD44 rat monoclonal (60068; StemCell Technologies, Inc., Vancouver, BC), tenascin C rabbit polyclonal (AB19011; EMD Millipore), fibronectin rabbit polyclonal (ab2413; Abcam), elastin mouse monoclonal (ab9519; Abcam),
Techniques: Quantitative RT-PCR, Western Blot, Produced